Aerolysin and pertussis toxin share a common receptor-binding domain

Aerolysin and pertussis toxin share a common receptor-binding domain.

We have discovered that the bacterial toxins aerolysin and pertussis toxin share a common domain. This is surprising because the two toxins affect cells in very different ways. The common domain, which we call the APT domain, consists of two three-stranded antiparallel beta-sheets that come together and wrap around a central pair of helices. The APT domain shares a common fold with the C-type l...

Thrombopoietin and neurotrophins share a common domain.

Dimer dissociation of the pore-forming toxin aerolysin precedes receptor binding.

The pore-forming toxin aerolysin is secreted by Aeromonas hydrophila as an inactive precursor. Based on chemical cross-linking and gel filtration, we show here that proaerolysin exists as a monomer at low concentrations but is dimeric above 0.1 mg/ml. At intermediate concentrations, monomers and dimers appeared to be in rapid equilibrium. All together our data indicate that, at low concentratio...

Cloning of Binding Domain of Clostridium botlllinum Toxin Type A in Escherichia coli

Identification of a receptor-binding domain of Bordetella dermonecrotic toxin.

Bordetella dermonecrotic toxin (DNT) stimulates the assembly of actin stress fibers and focal adhesions by deamidating or polyaminating Gln63 of the small GTPase Rho. DNT is an A-B toxin which is composed of an N-terminal receptor-binding (B) domain and a C-terminal enzymatically active (A) domain. In this study, to analyze the functional and structural organization of DNT, we prepared 10 clone...